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| Registros recuperados: 12 | |
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| Leite,Ricardo de Miranda Henriques; Thompson,James Alan; Gonçalves,Vítor Salvador Picão; Leite,Rômulo Cerqueira; Bandeira,Dimas A.; Lage,Andrey Pereira. |
| A serosurvey was conducted to estimate the prevalence of bovine brucellosis in the State of Paraíba, Brazil. A random sample of six municipalities was selected from each of three ecoregions of the State: the Agreste/Litoral region (1), the Cariri/Curimatau region (2) and the Sertão region (3). These 18 municipalities were divided in quadrants and one herd was selected at random from each quadrant. The sampling scheme included herds with more than 50 bovines, which excludes small-holder subsistence livestock production. At least 32 animals were sampled within each herd, stratified in four age categories, as follows: (1) at least 8 animals under 6 months of age, (2) at least 8 animals of 7 to 18 months of age, (3) at least 8 animals of 19 to 30 months of age... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Brucellosis; Epidemiology; Cattle; Paraíba; Brazil. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95962003000900002 |
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| Miranda,Karina Leite; Lage,Andrey Pereira. |
| The antimicrobial susceptibility of 25 Campylobacter sp strains isolated from calves with and without diarrhea - 7 C. coli, 16 C. fetus and 2 C. jejuni was studied by the disk diffusion method. Eleven antimicrobial agents were tested amikacin, ampicillin, kanamycin, chloramphenicol, erythromycin, gentamicin, neomycin, nitrofurantoin, penicillin G, tetracycline and sulfamethoxazole-trimethoprim. All Campylobacter sp strains were susceptible to amikacin, ampicillin, chloramphenicol, erythromycin, gentamicin, neomycin and nitrofurantoin. Three strains were moderately susceptible to kanamycin (2 C. coli and 1 C. fetus). All the strains were resistant to penicillin G. Two C. fetus strains were moderately susceptible to sulfamethoxazole-trimethoprim and 1 C.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Campylobacter sp; Calf; Diarrhea; Antimicrobial agents; Susceptibility. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000200032 |
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| Reis,Luciana Pimenta; Menezes,Liliane Denize Miranda; Lima,Graciela Kunrath; Santos,Ethiene Luiza de Souza; Dorneles,Elaine Maria Seles; Assis,Débora Cristina Sampaio de; Lage,Andrey Pereira; Cançado,Silvana de Vasconcelos; Figueiredo,Tadeu Chaves de. |
| ABSTRACT: In order to detect and identify Campylobacter spp. in broiler chicken carcasses, and to compare detection methods, 43 chilled and 43 frozen carcasses were collected and analyzed. Three methodologies were evaluated: an automated Enzyme Linked Fluorescent Assay (ELFA) VIDAS®30, Polymerase Chain Reaction (PCR) and real-time PCR. Only four chilled carcasses (4.6%) were considered positive for Campylobacter spp. by VIDAS®30 and no sample was positive when the conventional PCR technique was used. However, real-time PCR showed a higher incidence of contamination by Campylobacter spp. in broiler carcasses, with 45 (52.3%) positive samples. C. jejuni was the species most frequently reported in the samples (88.8%). No differences in the frequencies of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: C. jejuni; C. coli; Detection methods; VIDAS®30.. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018000200451 |
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| Freitas,Moisés Dias; Ferreira,Marina Guimarães; Ferreira,Paulo Marcos; Carvalho,Antônio Último; Lage,Andrey Pereira; Heinemann,Marcos Bryan; Facury Filho,Elias Jorge. |
| O conhecimento sobre a fisiologia e regulação dos eletrólitos e do equilíbrio ácido-base é necessário, pois a saúde do animal depende diretamente da composição normal dos fluídos nos diversos compartimentos corporais. Esta regulação está intimamente entrelaçada em um rigoroso e preciso controle, de modo que uma anormalidade em um destes compartimentos reflete na alteração dos demais. A avaliação do equilíbrio eletrolítico e ácido-base pode fornecer informações fundamentais para o diagnóstico, prognóstico e decisão terapêutica de diversas enfermidades nos bovinos, tais como diarreias neonatais, hemoparasitoses (anaplasmose e a babesiose), deslocamento de abomaso e processos de sépticos. As correções destas alterações com medidas terapêuticas adequadas são... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Equilíbrio ácido-base; Equilíbrio eletrolítico; Bovinos. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782010001200028 |
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| Silva,Teane Milagres Augusto da; Oliveira,Raquel Gonzaga de; Mol,Juliana Pinto da Silva; Xavier,Mariana Noyma; Paixão,Tatiane Alves da; Cortez,Adriana; Heinemann,Marcos Bryan; Richtzenhain,Leonardo José; Lage,Andrey Pereira; Santos,Renato de Lima. |
| Infectious abortion is a significant cause of reproductive failure and economic losses in cattle. The goal of this study was to detect nucleic acids of several infectious agents known to cause abortion including Arcanobacterium pyogenes, Bovine Herpesvirus 1, Brucella abortus, Campylobacter fetus subsp. venerealis, Chlamydophila abortus, Leptospira sp., Listeria monocytogenes, Salmonella sp., Mycoplasma bovis, Mycoplasma bovigenitalium, Neospora caninum, and Tritrichomonas foetus. Tissue homogenates from 42 fetuses and paraffin-embedded tissues from 28 fetuses and 14 placentas/endometrium were included in this study. Brucella abortus was detected in 14.2% (12/84) of the samples. Salmonella sp. DNA was amplified from 2 fetuses, and there was one positive... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cattle; Abortion; Brucella abortus; Salmonella sp.; Neospora caninum; Listeria monocytogenes. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782009000900028 |
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| Barbosa,Edel Figueiredo; Figueiredo,Henrique César Pereira; Garcia,Adriana Melo; Lobato,Zélia Inês Portela; Lage,Andrey Pereira. |
| A freqüência de rotavírus do grupo A foi avaliada em 194 amostras, de fezes de bezerros, com idade entre 2 e 60 dias, provenientes de 33 propriedades da bacia leiteira de Pará de Minas, Minas Gerais. Utilizando um sistema de ELISA monoclonal direto, 33 (17%) destas amostras foram positivas para rotavírus do grupo A. Do total de animais positivos para rotavírus do grupo A, 60,6% apresentavam diarréia no momento da coleta. Das 33 propriedades amostradas, a presença de rotavírus foi detectada em 19 (57,6%). Dentre todas as amostras examinadas, somente 7 apresentaram simultaneamente rotavírus e Cryptosporidium spp. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Rotavírus do grupo A Cryptosporidium spp. bezerros diarréia Minas Gerais; Brasil. |
| Ano: 1998 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84781998000300014 |
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| Dorneles,Elaine Maria Seles; Pellegrin,Aiesca Oliveira; Péres,Igor Alexandre Hany Fuzeta Schabib; Mathias,Luis Antônio; Mourão,Guilherme; Bianchi,Rita de Cassia; Olifiers,Natalie; Rocha,Fabiana Lopes; Lage,Andrey Pereira. |
| A serological survey in free-ranging crab-eating foxes (Canidae: Cerdocyon thous) and brown-nosed coatis (Procyonidae: Nasua nasua) was performed in the Nhecolândia sub-region of the Brazilian Pantanal to evaluate the presence of anti-smooth Brucella antibodies on those wild populations. The detection of anti-smooth Brucella antibodies was performed by the Rose Bengal Test (RBT) as screening test and the Fluorescence Polarization Assay (FPA) as a confirmatory test. The frequency of smooth Brucella seropositive animals were 13.2% (5/38, 95% CI: 4.4% - 28.1%) for crab-eating foxes and 8.8% (3/34, 95% CI: 1.9% -23.7%) for brown-nosed coatis. No association was found between seropositivity for brucellosis and gender or age. The results of this study suggest... |
| Tipo: Info:eu-repo/semantics/report |
Palavras-chave: Brazil; Brucella; Pantanal; Cerdocyon thous; Nasua nasua; Reservoirs; Epidemiology. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782014001202193 |
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| Herrmann,Geder Paulo; Lage,Andrey Pereira; Moreira,Elvio Carlos; Haddad,João Paulo Amaral; Resende,José Renato de; Rodrigues,Rogério Oliveira; Leite,Rômulo Cerqueira. |
| A presença de aglutininas anti-Leptospira spp. em 1360 amostras soros de ovinos, clinicamente sadios com mais de um ano de idade, criados extensivamente em 136 fazendas de 18 municípios; 10 na Mesorregião Sudeste e 8 na Mesorregião Sudoeste do Estado do Rio Grande do Sul, entre os meses de janeiro a março de 1999. Foi determinado pela Técnica de Aglutinação Microscópica (MAT), das 1360 amostras de soros testados, 466 (34,26%) animais foram reagentes e os títulos de aglutininas anti-Leptospira spp. variavam de 100 a 3200. As sorovariedades encontradas foram: hardjo (Norma), 210 (28,4%), sentot, 152 (16,8%); hardjoprajitno, 133 (14,5%); fortbragg 73 (6,3%), wolffi, 39 (4,7%); pyrogenes, 25 (1,8%); australis, 21 (1,6%); pomona, 20 (1,6%); sejroe, 19 (2,2%);... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Leptospirose; Ovino; Prevalência; Rio Grande do Sul. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782004000200017 |
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| Sales,Mariana L.; Fonseca Júnior,Antônio Augusto; Orzil,Lívia; Alencar,Andrea Padilha; Silva,Marcio Roberto; Issa,Marina Azevedo; Soares Filho,Paulo Martins; Lage,Andrey Pereira; Heinemann,Marcos Bryan. |
| Mycobacterium tuberculosis is the major cause of tuberculosis in humans. This bacillus gained prominence with the occurrence of HIV, presenting itself as an important opportunistic infection associated with acquired immunodeficiency syndrome (AIDS). The current study aimed to develop a real-time PCR using Eva Green technology for molecular identification of M. tuberculosis isolates. The primers were designed to Rv1510 gene. Ninety nine samples of M. tuberculosis and sixty samples of M. bovis were tested and no sample of the bovine bacillus was detected by the qPCR. Statistical tests showed no difference between the qPCR and biochemical tests used to identify the Mycobacterium tuberculosis. The correlation between tests was perfect with Kappa index of 1.0... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Mycobacterium tuberculosis; Diagnosis; Real time PCR. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000400029 |
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| Coura,Fernanda Morcatti; Diniz,Soraia de Araújo; Silva,Marcos Xavier; Oliveira,Cairo Henrique Sousa de; Mussi,Jamili Maria Suhet; Oliveira,Camila Stefanie Fonseca de; Lage,Andrey Pereira; Heinemann,Marcos Bryan. |
| ABSTRACT: This study aimed to determine the virulence factors, phylogenetic groups, and the relationships between pathovars and phylogenetic groups of E. coli strains isolated from feces of buffalo calves. A total of 217 E. coli strains were obtained from feces after culture and were screened by PCR for detection of virulence factors EAST-1, enterohemolysin, Saa, CNF2, F41, F5, STa, intimin, Stx1 and Stx2. One hundred and thirty-four isolates were positive for one or more virulence factors: eighty-four from diarrheic animals, and fifty from non-diarrheic calves. The pathovars of E. coli identified in diarrheic feces were ETEC (F5+) (2/84), NTEC (16/84), STEC (20/84), EPEC (3/84), EHEC (3/84), and EAEC (EAST-1+) (33/84). Pathovars identified in... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Buffalo calves; Diarrhea; Escherichia coli; Pathovars; Phylogenetic group. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782019000500451 |
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| Registros recuperados: 12 | |
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